On November 8th, Amazon Studios reported it would open an investigation into Transparent actor Jeffrey Tambor after becoming aware of sexual misconduct allegations made by his former assistant, Van Barnes. The Emmy and Golden Globe-winner denied those allegations. Now Tambor is again denying sexual harassment claims made on November 16th by his Transparent co-star, Trace Lysette.

In the groundbreaking Amazon show, Tambor plays Maura, a newly transitioned male-to-female transgender person and father (or “MaPa,” the term of endearment used on the show). Lysette plays one of Maura’s friends who teaches her how to take control of her womanhood.

Lysette claims that Tambor made an unwanted sexual advance toward her in 2015 while waiting to begin shooting a scene in which they were both wearing pajamas.

She said this happened after Tambor announced, “My God, Trace. I want to attack you sexually,” after seeing Lysette in her semi-revealing costume.

Credit: Getty Images / Larry French

In a statement released to Us Weekly,Tambor denied these allegations. The 73-year-old actor stated,

Tambor previously called a statement made by Van Barnes a “baseless allegation.”

Amazon stated that Lysette’s allegations will be added to their ongoing “conversation” and investigation involving Tambor.